Using the Regenerator in conjunction with short range weapons leaves you almost invincible, though you will still be vulnerable to snipers.
A Regenerator is very useful for defending yourself against grenades. Some players believe they could survive a grenade spammer with a Regenerator, however, if two frag grenades are thrown in succession, they are enough to kill a player while standing inside a Regenerator's field.
Another tactic is a grenade followed with a quick headshot on a normally-shielded opponent.
In campaign, if a Brute throws a Regenerator, do not run into it, as it heals him too. All in all, do not run into an enemy's Regenerator just because it exists.
Use the Regenerator to quickly recover from an instant shield dropping blow. If in teams, it is best to drop the Regenerator and have somebody drop a Bubble Shield. If in Free For All, drop it in a hard to reach place, like one of the Shotgun corners in The Pit.
When in a Regenerator with an opponent, melee, don't shoot; it is a much more effective way to take down your opponents, unless you have a weapon such as a Shotgun, Sniper, or a Mauler (one-shot kill, or close to a one-shot kill).
Throwing a Regenerator inside a Bubble Shield while camping is a way to survive the grenade that is often dropped after a player's death.
A Regenerator does not recharge an Overshield; only the normal shielding.
Try using the Regenerator against enemy players on Narrows by throwing it in a Man Cannon (see trivia).
When playing King of the Hill gametypes, throwing down a Regenerator inside the hill can help you sustain control, but can ultimately lead to many contentions for the hill by multiple players constantly having full shields.